

I don’t remember that much, I think it is something about temperature - the -80°C freezers just freeze the culture much faster so you don’t kill the cells. You can mitigate it by using glycerol as antifreeze but it is bit more work.
With agar you store only the pure 1st gen of the yeasts so there is bit more work after you unfreeze them. So there is probably different method to freezing yeast cake.
When I messed with the yeast I used this playlist as instructions:
https://www.youtube.com/playlist?list=PLZ3Z2428mCTqDFK-3ZSNpZBXJfTaMtRXE
There is something about the storing of the yeasts (I think they can last years frozen).


















Avatar is just playground for CGI without any deep or remembare story!
When I say that I just don’t exactly like this type of movie and I just don’t care about it more I got a bit laughed at.